Nematodes of zoonotic importance in Cynoscion guatucupa (Pisces) in the state of Rio de Janeiro / Nematóides de importância zoonótica em Cynoscion guatucupa (Pisces) no estado do Rio de Janeiro

Between January and August 2012, thirty specimens of Cynoscion guatucupa (Cuvier, 1830) caught off coast of the municipality of Itajaí, state of Santa Catarina, Brazil (26° 54' 28″ S and 48° 39' 43″ W) and commercialized in the state of Rio de Janeiro were investigated for the presence of zoonotic nematodes. In total 853 larvae of four nematode parasite species were found. There were three species of Anisakidae: three larvae of Anisakis sp., with prevalence (P) of 10%, mean intensity (MI) of 1.0, mean abundance (MA) of 0.1 and infection site (IS) in the mesentery; five of Terranova sp. with P = 13.3%, MI = 1.25, MA = 0.17, range of infection (RI) from 1 to 2 and IS = mesentery; and seven of Contracaecum sp. with P = 6.6%, MI = 3.5, MA = 0.23, RI = 1 to 4 and IS = mesentery and abdominal cavity. There was one species of Raphidascarididae: 838 larvae of Hysterothylacium deardorffoverstreetorum with P = 83.3%, MI = 33.52, MA = 27.93, RI = 1 to 219 and IS = mesentery, liver serosa and abdominal cavity. This is the first report of larvae of Anisakis sp. and Contracaecum sp in C. guatucupa in Brazil.

Entre janeiro e agosto de 2012, 30 espécimes de Cynoscion guatucupa (Cuvier, 1830) pescados no litoral do município de Itajaí, estado de Santa Catarina, Brasil (26° 54' 28″ S e 48° 39' 43″ W) e comercializados no estado do Rio de Janeiro, foram investigados quanto á presença de nematóides zoonóticos. Foram encontradas 853 larvas de quatro espécies de nematóides parasitos. Três espécies pertencem á Anisakidae, sendo três larvas de Anisakis sp., com prevalência (P) de 10%, intensidade média (IM) de 1, abundância média (AM) de 0,1 e sítio de infecção (SI) o mesentério; cinco de Terranova sp., com P = 13,3%, IM = 1,25, AM = 0,17, amplitude de variação da intensidade de infecção (AI) de 1 a 2 e SI = mesentério; e sete de Contracaecum sp., com P = 6,6%, IM = 3,5, AM = 0,23, AI = 1 a 4 e SI = mesentério e cavidade abdominal. Uma espécie pertence á Raphidascarididae, sendo 838 larvas de Hysterothylacium deardorffoverstreetorum com P = 83,3%, IM = 33,52, AM = 27,93, AI = 1 a 219 e SI = mesentério, serosa do fígado e cavidade abdominal. Este é o primeiro registro de larvas de Anisakis sp. e Contracaecum sp. em C. guatucupa no Brasil.

Myxidium volitans sp. nov., a parasite of the gallbladder of the fish, Dactylopterus volitans (Teleostei: Triglidae) from the Brazilian Atlantic coast: morphology and pathology

Myxidium volitans sp. nov. (Myxozoa: Myxidiidae) parasitizing the hypertrophied green-brownish gallbladder of the teleost Dactylopterus volitans, collected in the Atlantic coast near Niterói, Brazil was described based on ultrastructural studies. The spores were fusiform, sometimes slightly crescent-shaped on average 21.7 ± 0.3 µm (mean ± standard deviation) (n = 50) long and 5.6 ± 0.4 µm (n = 30) wide. The spore wall was thin and smooth, comprising two equally-sized valves joined by a hardly visible sutural ridge. Spores containing two pyriform polar capsules (PC) (5.0 ± 0.4 × 2.3 ± 0.3 µm) (n = 30) are situated in each extremity of the spore. The PC wall was composed of hyaline layer (0.20-0.29 µm thick) and by a thin external granular layer. Each PC contains a polar filament (PF) with irregular arrangements that was projected from its apical region to the bases of PC and coiled laterally from bases to the tip of PC. Some regular striations and S-like structures in the periphery of the PFs with four-five irregular sections were observed. Based on the spore morphology, ultrastructural differences and the specificity of the host we describe this parasite as a new myxosporidian, named M. volitans sp. nov.

Registro de lynxacariose em Felis catus domesticus na cidade de Jundiaí, Estado de São Paulo / Occurrence of lynxacariose in Felis catus domesticus in Jundiaí city, State of São Paulo

Ácaros parasitas de pêlos de felino examinado na cidade de Jundiaí, Estado de São Paulo, foram identificados no Laboratório de Morfofisiologia e Patogenia de Carrapatos (LMPC) da Universidade Federal Rural do Rio de Janeiro (UFRRJ) como a espécie Lynxacarus radovskyi, constituindo o primeiro registro desta espécie de parasito no Estado de São Paulo.

Mites from a cat fur examined in Jundiaí city, State of São Paulo, were identified at Tick Morfofisiology and Pathogeny Laboratory (UFRRJ) as Lynxacarus radovskyi. This is the first occurrence of the species registred in south-eastern Brazil.

Um novo meio de transporte e cultivo para Tritrichomonas foetus (Riedmuller, 1928) V. Lactopep como meio de cultivo / A new transfer and culture medium of Tritrichomonas foetus (Riedmuller, 1928) V. Lactopep as a culture medium

Para o diagnóstico da tricomonose bovina, o meio Lactopep foi testado para o transporte de amostras de lavado prepucial e de muco vaginal de bovinos infectados com Tritrichomonas foetus. A metodologia de colheita do material foi descrita por Lopes et al. (1994), utilizando o meio Guida-Kupferberg como testemunho. Os meios foram mantidos em temperatura ambiente e analisados após 24 horas. Pela análise de variação com delineamento inteiramente casualizado, avaliou-se o dia de máxima eficiência. Com Lactopep obteve-se a média de 9,8 dias e com Guida-Kupferberg, 9,2 dias, com material proveniente de machos naturalmente infectados; as médias das culturas para material proveniente de fêmeas foi 7,1 dias para o Lactope, e de 7,5 para o Guida-Kupferberg. A estatística demonstrou que os meios são eficientes para o transporte e cultivo de T. foetus, mas o meio Lactopep é de mais fácil preparo e de baixo custo. Na segunda etapa do experimento, adicionou-se ao Lactopep a fração de 10% de soro fetal bovino para manutenção de T. foetus por repiques sucessivos em laboratório, comparando-se ao de Hanks. Os dois meios permitem curva de crescimento em torno de sete dias, quando a fase de esgotamento chega aos valores mais baixos mas ainda viáveis. O Lactopep modificado para laboratório também mantém acentuada correlação negativa com o tempo de cultivo e pode ser indicado para a manutenção de T. foetus.

Lactopep medium efficacy for transport of Tritrichomonas foetus was checked in sample obtained from preputials washing of bulls and vaginal mucus of cows knowghy T. foetus positive, and compared to Guida-Kupferberg medium. Samples were obtained from bulls or cows with 0,85% saline solution and, half part of sample received three grams Lactopep; to the other half part, three grams of Guida-Kupferberg medi um were added. Both groups were kept at environmental temperature and daily examined under microscopy. The results were statistically analised by a variance analisis for casualised entirely delineation surveying the efficient maximum day. The Lactopep medium and Guida-Kupferber medium mean proceeded 9.8 day and 9.2 day respectively, with material from bulls, and 7.1 day and 7.5 day, respectively, with material from cows, and the conclusion is that both media are efficient for T. foetus maintenance but Lactopep medium has the advantage of the easy preparation and is produced at lower cost. In second stage to experiment to added in Lactopep medi um the fraction of 10% bovine se rum for maintainance to T. foetus by sucessive tollings in laboratory; the medium was compared with the Hanks medium. The results showed the both media allowed a line arround the seven days at the declive but further practicable. The Lactopep medium modificated for laboratory also conserve accentuated invertion correlation with the time cultivation, but it is indicat for maintaince of T. foetus in laboratory.